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Research Associate - Metabolic Engineering

Phycus Biotechnologies

Phycus Biotechnologies

Toronto, ON, Canada
Posted on Oct 27, 2025
We are currently seeking a research associate to support projects related to metabolic engineering. This position will focus on chemical production in the host organism and the candidate is expected to have a strong background in metabolic engineering, molecular biology, genetics, and ideally synthetic biology. Strong technical, organizational, and communication skills, as well as the ability to develop creative solutions to challenging problems, are essential.Immediate responsibilities include:1. Genetic design of strains to produce desired compounds in engineered microbes.2. Optimize the cultivation condition and assays for strain screening3. Routinely perform molecular biology techniques such as primer design, PCR, DNA and RNA extraction, DNA mutagenesis, DNA cloning, plasmid mini-prep, DNA sequence analysis, cell transformation, cell culture, and other more advanced molecular biology techniques in support of research projects.4. Perform experiments for enzymatic activity testing including express proteins in coli and yeast, recombinant proteins purification; analyze expressed proteins by SDS-PAGE and/or in vitro / in vivo enzymatic assays.5. Operate laboratory and experimental equipment such as spectrophotometers, centrifuges, HPLC, LC/MS and fermentation reactors.6. Work collaboratively with other team members to accomplish project goals and milestones.7. Routinely track and document results in laboratory notebooks and databases.8. Analyze, summarize, and present experimental results to team leader and other team members.Qualifications:1. Master’s Chemical Engineering, Bioprocess Engineering, Microbiology or related field, required direct relevant fermentation experience.2. Experience with standard molecular biology techniques (PCR, DNA extraction, mini-prep, primer design)3. Experience with design, development, optimization, scale-up, and/or technology transfer of industrial fermentation-based production processes.4. Experience developing fermentation processes for both bacteria and yeast5. Advanced process modeling capabilities (Excel, Python, Aspen, Matlab, etc.).6. Strong mechanical aptitude and knowledge of fermentor control hardware/software.7. Proven ability to communicate effectively both orally and in writing.8. Strong analytical skills, attention to detail, and strong computer skills (Microsoft Office, etc.).This position is based in Toronto. Competitive salary and employee stock options.